General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR65 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0428E2ACP1L)
The PathHunter GPR65 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR65 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR65 and EA-tagged β-Arrestin-2. Activation of GPR65 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRD CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0926E2CP2M)
The PathHunter MRGPRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRD and EA-tagged β-Arrestin-2. Activation of MRGPRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 2-Plate. (93-1045Y3-00043)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery R-PLEX Human -GST ASY
The R-PLEX Human Alpha GST Assay Kit from Meso Scale Discovery measures alpha glutathione S-transferase (GSTA) in human serum plasma and cell culture supernatants This ECL immunoassay uses MSD s SULFO-TAG labels and multi-array plate technology for sensitive rapid GSTA detection The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and necessary reagents Results are available in 3 5 hours with a 3-minute read time per plate The assay range is 3 2-10 000 pg/mL with no cross-reactivity with other proteins
American Research Products Inc Rat eXtracellular superoXide d
Rat eXtracellular superoXide dismutase [Cu-Zn] (Cu/Zn-SOD/SOD3) ELISA kit from CUSABIO Detection Range 15 6 pg/mL-1000 pg/mL Sensitivity 3 9 pg/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
Revvity Health Sciences Inc HTRF Human Phospho-EGFR (Tyr1068) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-EGFR (Tyr1068) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK4 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CDK4 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-STING (Ser366) Detection Kit, 500 Assay Points
HTRF Human Phospho-STING (Ser366) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total SHP1 Detection Kit, 500 Assay Points
HTRF Human Total SHP1 Detection Kit, 500 Assay Points
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (10 plate), detects SA-β-gal with high sensitivity by SPiDER-βGal, applicable to both living and fixed cells, by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Revvity Health Sciences Inc HTRF Total NDRG1 Detection Kit, 50,000 Assay Points
HTRF Total NDRG1 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total c-Src Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total c-Src Detection Kit, 10,000 Assay Points